SYBR Color qPCR SuperMix (Without ROX)

SYBR Color qPCR SuperMix is an optimized 2X real-time PCR master mix containing HotStarTaq DNA Polymerase, SYBR Green fluorescent dye, dNTPs, and Mg2+. The balanced ratio of K+ and NH4+ ions in the buffer promotes specific primer annealing, ensuring high reaction sensitivity and specificity. Simply add primers and cDNA template to this ready-to-use master mix to initiate the reaction, greatly streamlining the workflow while minimizing contamination risks. The unique buffer formulation delivers sensitive qPCR performance across all real-time PCR platforms without requiring optimization. Additionally, an integrated color-change indicator visualizes template addition, significantly improving pipetting efficiency and accuracy.