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Tau-441 (2N4R) P301S Mutant Monomers
SKU: orb1822359
Featured
Description
Research Area
Cell Biology, Disease Biomarkers, Neuroscience, Signal Transduction
Images & Validation
−Item 1 of 2
| Tested Applications | In vitro, In vivo, SDS-PAGE, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | Recombinant |
|---|---|
| Expression System | E. coli |
| Biological Origin | Human |
| Biological Activity | Thioflavin T emission curve shows increased fluorescence (correlated to tau protein fibrillation) when tau PFFs are combined with tau monomers. |
| Target | Tau-441 (2N4R) P301S Monomers |
| Reactivity | Human |
| Tag | No tag |
| Molecular Weight | ~45.8 kDa |
| Protein Length | Full Length |
| Protein Sequence | MAEPRQEFEV MEDHAGTYGL GDRKDQGGYT MHQDQEGDTD AGLKESPLQT PTEDGSEEPG SETSDAKSTP TAEDVTAPLV DEGAPGKQAA AQPHTEIPEG TTAEEAGIGD TPSLEDEAAG HVTQARMVSK SKDGTGSDDK KAKGADGKTK IATPRGAAPP GQKGQANATR IPAKTPPAPK TPPSSGEPPK SGDRSGYSSP GSPGTPGSRS RTPSLPTPPT REPKKVAVVR TPPKSPSSAK SRLQTAPVPM PDLKNVKSKI GSTENLKHQP GGGKVQIINK KLDLSNVQSK CGSKDNIKHVSGGGSVQIVY KPVDLSKVTS KCGSLGNIHH KPGGGQVEVK SEKLDFKDRV QSKIGSLDNI THVPGGGNKK IETHKLTFRE NAKAKTDHGA EIVYKSPVVS GDTSPRHLSN VSSTGSIDMV DSPQLATLAD EVSASLAKQG L |
| Purification | Ion-exchange Purified |
| Purity | >95% |
Storage & Handling
−| Storage | -80°C |
|---|---|
| Buffer/Preservatives | 10 mM HEPES, 100 mM NaCl pH 7.4 |
| Concentration | 2 mg/ml |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−Tau monomer, Tau protein monomer, Tau protein, microtubule-associated protein Tau, MAPT, MAP, microtubule-associated protein, Tau-441, Paired Helical Filament-Tau, Phf-Tau, Neurofibrillary Tangle Protein, G Protein Beta1/Gamma2 Subunit-Interacting Factor 1, Isoform 2, tubulin-associated unit
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SDS-PAGE of ~67 kDa Human Tau Protein 2N4R P301S Monomer. Lane 1: MW ladder. Lane 2: Tau Protein Monomer
Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in tau fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) over time in tau monomers. A greater increase in fluorescence is seen when 50 uM monomer is combined with 10 nM PFFs, as the fibrils seed the formation of new fibrils from the pool of monomers. Thioflavin T ex = 450 nm, em = 485 nm. 10 uM heparin was added to each well.
Quick Database Links
Gene Symbol
Tau-441 (2N4R) P301S Monomers
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
Tau-441 (2N4R) P301S Mutant Monomers (orb1822359)
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