TBK1 Antibody (S172)

SKU: orb1928791

Description

Research Area

Immunology & Inflammation, Infectious Disease & Virology, Signal Transduction

Images & Validation

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Item 1 of 5

Tested Applications	IHC-P, WB
Dilution range	IHC-P - 1:100-500, WB - 1:2000
Reactivity	Human, Mouse
Predicted Reactivity	Xenopus

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Molecular Weight	83642 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Disclaimer	For research use only

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Formalin-fixed and paraffin-embedded human testis tissue reacted with TBK-pS172, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-TBK Antibody (S172) at 1:2000 dilution. Lane 1: HT-1080 whole cell lysate. Lane 2: K562 whole cell lysate. Lane 3: MCF-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 84 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-TBK1 Antibody (S172) at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: HT-1080 whole cell lysate. Lane 3: K562 whole cell lysate. Lane 4: MCF-7 whole cell lysate. Lane 5: THP-1 whole cell lysate. Lane 6: mouse testis lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 84kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining TBK in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Staining TBK1 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Quick Database Links

UniProt

Q9UHD2

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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