TBX21/T-bet Antibody

SKU: orb1535058

Description

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, IF, IHC, IHC-P, WB
Dilution Range	ELISA, IF (5 µg/ml), IHC, IHC-P (10 µg/ml), WB (1 µg/ml)
Reactivity	Human, Mouse
Application Notes	Further information: TBX21 antibody can be used for detection of TBX21 by Western blot at 1 - 2 ug/ml. Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 59 kDa Observed: 55kDa

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	A 19 amino acid peptide near the C-Terminus of human TBX2. The immunogen is located within the last 50 amino acids of TBX21 .
Target	TBX21 / T-bet
Purification	Peptide Column Affinity Chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS containing 0.02% Sodium Azide
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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TBX21, T-bet, TBET, T-box 21, T-box protein 21, TBLYM, Transcription factor TBLYM, T-box expressed in T cells, T-PET

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Anti-TBX21 / T-bet antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Immunofluorescence of TBX21 in 293 cells with TBX21 antibody at 5 ug/ml.

Western blot analysis of TBX21 in 293 cell lysate with TBX21 antibody at 1 ug/ml.

Quick Database Links

Gene Symbol

TBX21 / T-bet

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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IHC-P

Immunohistochemistry Paraffin

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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