TCF4 Antibody (Biotin)

SKU: orb413794

Description

Rabbit polyclonal antibody against TCF4 conjugated to Biotin

Images & Validation

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Tested Applications	IHC, WB
Dilution range	WB (1:1000); IHC (1:50)
Reactivity	Human, Mouse
Application Notes	A 1:1000 dilution of SPC-723 was sufficient for detection of TCF4 in 15 µg of mouse liver cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide from the C-terminal of Human TCF4 (500-600 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
Target	TCF4
Molecular Weight	71 kDa, 45 kDa
Purification	Peptide Affinity Purified
Conjugation	Biotin

Storage & Handling

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Storage	Conjugated antibodies should be stored according to the product label
Buffer/Preservatives	136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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TCF-4, MGC149723, PTHS, Class B basic helix-loop-helix protein 19, bHLHb19, SEF 2, SL3 3 enhancer factor 2, SEF2 1, ITF-2, E2 2, SEF2 1A, BHLHB19, MGC149724, ITF 2, Transcription factor 4, TCF 4, SEF-2, SEF2 1B, Immunoglobulin transcription factor 2, SL3-3 enhancer factor 2, SEF2, ITF2, ITF2_HUMAN

Phospho-HNF4 (Ser304) Rabbit Polyclonal Antibody (Biotin) [orb502507]

Applications:	FC, IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:	Bovine, Equine, Gallus, Guinea pig, Porcine, Rabbit, Rat, Zebrafish
Reactivity:	Human, Mouse
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Biotin

Sizes Available: 100 μl

TCF4 Rabbit Polyclonal Antibody (Biotin) [orb2065984]
Applications:
WB
Predicted Reactivity:
Equine, Guinea pig, Human, Mouse
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Biotin
Sizes Available: 100 μl

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Immunohistochemistry analysis using Rabbit Anti-TCF4 Polyclonal Antibody. Tissue: Kidney. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-TCF4 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.

Western blot analysis of Mouse liver lysate showing detection of ~71.3 kDa TCF4 protein using Rabbit Anti-TCF4 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse liver lysate. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TCF4 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~71.3 kDa. Other Band (s): ~45 kDa isoform.

Quick Database Links

Gene Symbol

TCF4

UniProt

P15884

NCBI Gene ID

6925

RefSeq (Protein):NP_001077431.1

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_001077431.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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