TEM1/CD248 Antibody

SKU: orb1537860

Description

TEM1/CD248 Antibody

Images & Validation

−

Item 1 of 2

Tested Applications	ELISA, IF, IHC, IHC-P, WB
Dilution range	ELISA, IF, IHC, IHC-P (5 µg/ml), WB (0.5 - 1 µg/ml)
Reactivity	Human, Mouse, Rat
Application Notes	Further information: TEM1 antibody can be used for detection of TEM1 by Western blot at 0.5 - 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 48, 83 kDa Observed: 50, 88 kDa

Key Properties

−

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	A 14 AA synthetic peptide near the C-Terminus of human TEM1. The immunogen is located within the last 50 AA of TEM1.
Target	TEM1 / CD248
Purification	Peptide Column Affinity Chromatography
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS containing 0.02% Sodium Azide. PBS containing 0.02% Sodium Azide
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

−

CD248, CD248 antigen, CD164 sialomucin-like 1, CD248 antigen, endosialin, CD248 molecule, endosialin, Endosialin, TEM1, Tumor endothelial marker 1, CD164L1

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Immunofluorescence of TEM1 in Human Colon cells with TEM1 antibody at 20 ug/ml.

Western blot of TEM1 in human colon tissue lysate with TEM1 antibody at (A) 0.5 and (B) 1 ug/ml. Below: Immunohistochemistry of TEM1 in human colon tissue with TEM1 antibody at 2.5 ug/ml.

Quick Database Links

Gene Symbol

TEM1 / CD248

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC

Immunohistochemistry

View Protocol

IHC-P

Immunohistochemistry Paraffin

View Protocol

Immunofluorescence

View Protocol

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols