Terminal Transferase (TdT)

SKU: orb2975446

Description

Terminal transferase (TdT) is a template independent polymerase that catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules. It catalyzes the repeated addition of deoxyribonucleotides to oligodeoxyribonucleotides and 3' -Oh of single - and double-stranded DNA. TdT requires an oligodeoxynucleotide of at least three bases as a primer. The addition of Co2+ in the reacton makes tailing more efficient.

Images & Validation

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Key Properties

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Biological Origin	Bovine
Molecular Weight	45 kDa
Protein Length	Terminal Transferase (TdT) is expressed in E. coli.
Purity	>90% as determined by SDS-PAGE.
Endotoxins	Please contact with the lab for this information.

Storage & Handling

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Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.
Form/Appearance	Liquid
Buffer/Preservatives	100mM KAc (pH6.8), 2mM 2-mercaptoethanol, 0.01%Triton X-100 and 50% glycerol.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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DNA nucleotidylexotransferase, 2.7.7.31, Terminal addition enzyme, Terminal deoxynucleotidyltransferase, TDT, Terminal transferase, DNTT, TDT

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