TFE3 Antibody / Transcription factor E3

SKU: orb1822638

Description

Transcription factor E3 (TFE3) belongs to the basic helix-loop-helix zipper transcription factor family. The TFE3 gene is located on the short arm of the X chromosome, is expressed in various cells of the human body, and participates in the regulation of various genes. The rearrangement of this gene is associated with a variety of tumors, and it is highly expressed in acinar soft tissue sarcoma with TFE3 fusion gene, Xp11.2 translocation/TFE3 gene fusion-related renal cell carcinoma, and in perivascular epithelioid cell tumors. positive expression.

Images & Validation

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Tested Applications	IHC-P
Dilution Range	Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Reactivity	Human
Application Notes	Optimal dilution of the TFE3 antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	TFE3/6849R
Immunogen	Recombinant full-length human TFE3 protein was used as the immunogen for the TFE3 antibody.
Purification	Protein A/G affinity
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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IHC staining of FFPE human MiT translocation RCC with TFE3 antibody. Nuclear staining using TFE3/6849R mAb at 2 ug/ml. HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

Negative control: IHC testing of FFPE human clear cell RCC tissue with TFE3 antibody (clone TFE3/6849R) at 2 ug/ml. HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

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UniProt

P19532

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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