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TGFBR2 Antibody (N-term)
SKU: orb1937905
Description
Research Area
Cardiovascular Research, Signal Transduction
Images & Validation
−Item 1 of 7
| Tested Applications | IF, IHC-P, WB |
|---|---|
| Dilution Range | WB - 1:2000 |
| Reactivity | Human, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 64568 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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All lanes: Anti-TGFBR2 Antibody (N-term) at 1:2000 dilution. Lane 1: human testis lysate. Lane 2: mouse lung lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 67 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-TGFBR2 Antibody (N-term) at 1:2000 dilution. Lane 1: NCCIT whole cell lysates. Lane 2: mouse lung lysates. Lane 3: SW620 whole cell lysates.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 67 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-TGFBR2 Antibody (N-term) at 1:2000 dilution + mouse lung lysates.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 65 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-TGFBR2 Antibody (N-term) at 1:2000 dilution + mouse lung lysates.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 67 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-TGFBR2 Antibody (N-term) at 1:2000 dilution + mouse lung lysates.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 67 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Confocal immunofluorescent analysis of TGFBR2 Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
TGFBR2 Antibody (N-term) western blot analysis in mouse lung tissue lysates (35 ug/lane). This demonstrates the TGFBR2 antibody detected the TGFBR2 protein (arrow).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
TGFBR2 Antibody (N-term) (orb1937905)
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