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THBS1 Antibody (N-term)
Description
Research Area
Images & Validation
−| Tested Applications | FC, IF, IHC-P, WB |
|---|---|
| Dilution Range | WB - 1:1000 |
| Reactivity | Human |
| Predicted Reactivity | Bovine |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 129383 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Western blot analysis of THBS1 Antibody (N-term) in MDA-MB231 cell line lysates (35 ug/lane). THBS1 (arrow) was detected using the purified Pab.

Confocal immunofluorescent analysis of THBS1 Antibody (N-term) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

THBS1 Antibody (N-term) flow cytometric analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with THBS1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Protocol Information
THBS1 Antibody (N-term) (orb2649271)
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