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Tie-2 (phospho Tyr1108) rabbit pAb Antibody
SKU: orb770239
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Description
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−Item 1 of 4
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution range | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Reactivity | Human, Mouse |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human TIE2 around the phosphorylation site of Tyr1108. AA range:1074-1123 |
| Molecular Weight | 150 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-TEK antibody, anti-TIE2 antibody, anti-VMCM antibody, anti-VMCM1 antibody, anti-Angiopoietin-1 receptor antibody, anti-Endothelial tyrosine kinase antibody, anti-Tunica interna endothelial cell kinase antibody, anti-Tyrosine kinase with Ig and EGF homology domains-2 antibody, anti-Tyrosine-protein kinase recept antibody
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using TIE2 (Phospho-Tyr1108) Antibody.
Immunofluorescence analysis of HepG2 cells, using TIE2 (Phospho-Tyr1108) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from NIH/3T3 cells, using TIE2 (Phospho-Tyr1108) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Tie-2 (phospho Tyr1108) rabbit pAb Antibody (orb770239)
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