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Tie-2 (phospho Tyr992) rabbit pAb Antibody
SKU: orb767261
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Description
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| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution range | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Reactivity | Human, Mouse |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | Synthesized phospho-peptide around the phosphorylation site of human Tie-2 (phospho Tyr992) |
| Molecular Weight | 125 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-TEK antibody, anti-TIE2 antibody, anti-VMCM antibody, anti-VMCM1 antibody, anti-Angiopoietin-1 receptor antibody, anti-Endothelial tyrosine kinase antibody, anti-Tunica interna endothelial cell kinase antibody, anti-Tyrosine kinase with Ig and EGF homology domains-2 antibody, anti-Tyrosine-protein kinase recept antibody
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Western Blot analysis of VEC cells using Phospho-Tie-2 (Y992) Polyclonal Antibody.
Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Western Blot analysis of mouse lung, mouse liver , using primary antibody at 1:1000 dilution. Secondary antibody was diluted at 1:10000.
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WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Tie-2 (phospho Tyr992) rabbit pAb Antibody (orb767261)
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