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Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 3
| Tested Applications | FC, IHC-P, WB |
|---|---|
| Dilution Range | WB - 1:1000, IHC-P - 1:10-50, FC - 1:10-50 |
| Reactivity | Human |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | BTD24625X24626 |
| Immunogen | This TLR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 212-242 amino acids from the N-terminal region of human TLR2. Antigen Region: 212-242 aa. |
| Target | TLR2 (HGNC:11848) |
| Molecular Weight | 89838 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Toll-like receptor 2, Toll/interleukin-1 receptor-like protein 4, CD282, TLR2, TIL4
Quality Guarantee
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Flow cytometric analysis of CEM cells using TLR2 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Anti-TLR2 Antibody (C226) at 1:1000 dilution + Ramos whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 90 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with TLR2 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_003255.2 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
TLR2 Antibody (N-term) (orb1935286)
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