TLR7 Rabbit Polyclonal Antibody

SKU: orb100357

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Description

Research Area

Developmental Biology, Inflammation, Macrophage, Signal Transduction, TLR Signaling Pathway

Images & Validation

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Item 1 of 2

Tested Applications	WB
Dilution Range	WB=1:500-2000
Reactivity	Human

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human TLR7 (461-560/1049aa)
Target	TLR7
Molecular Weight	121 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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TLR-7; PRO285; TLR 7; TLR7_HUMAN; Toll like receptor 7; Toll-like receptor 7; UNQ248.

Quality Guarantee

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Sample: Brain (Mouse) Lysate at 40 ug, Spleen (Mouse) Lysate at 40 ug, Primary: Anti-TLR7 (orb100357) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 121 kD, Observed band size: 121 kD.

Sample: Lane 1: Human THP-1 (LPS) cell lysates, Lane 2: Human THP-1 cell lysates, Lane 3: Human Jurkat cell lysates, Lane 4: Human MCF-7 cell lysates, Lane 5: Human 293T cell lysates, Primary: Anti-TLR7 (orb100357) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 121 kDa, Observed band size: 65, 121 kDa.

Quick Database Links

Gene Symbol

TLR7

UniProt

Q9NYK1

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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