TNF-alpha multimeric Recombinant Protein

SKU: orb1230176

Description

Research Area

Cancer Biology, Cell Biology, Immunology & Inflammation, Metabolism Research, Signal Transduction

Images & Validation

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Application Notes	This recombinant proteins is for research use only.

Key Properties

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Source	HEK293 cells
Biological Activity	Activates human and mouse TNF-R1 and TNF-R2. Induces cell death at a concentration range of 0.01-10ng/ml (ED50= 0.05ng/ml or 2 x 10E7 Units/mg; WEHI 164 cells).
Target	TNF
Tag	DDDDK Tag
Protein Sequence	The extracellular domain of human TNF-alpha (aa 85-233) is fused at the N-terminus to human ACRP30headless (aa 16-108) and a DDDDK-tag.
Purity	>95% (SDS-PAGE). Endotoxin level is less than 0.02EU/ μg purified protein (LAL test; Lonza).

Storage & Handling

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Storage	Stable for at least 6 months after receipt when stored at -20°C.
Form/Appearance	Lyophilized
Buffer/Preservatives	Contains PBS. Reconstitute with 100 μl sterile water.
Concentration	0.1mg/ml after reconstitution.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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ACRP30headless:TNF-alpha, ACRP30headless:TNFSF2, ACRP30headless:Tumor Necrosis Factor-alpha

Quality Guarantee

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Quick Database Links

Gene Symbol

TNF

UniProt

P01584

RefSeq:P01375

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	P01375

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