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Description
Research Area
Immunology & Inflammation
Images & Validation
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Item 1 of 1
| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human TNF-alpha at 5 ug/mL (100 ul/well) can bind Humira with a linear range of 0.04-0.6 ng/mL. The bio-activity was determined by its cytotoxcity assy using L929 Cells with 1ug/ml actinomycin D. |
| Target | TNF |
| Tag | Tag free |
| MW | 17.4 kDa |
| Purity | >95% as determined by SDS-PAGE. |
| Protein Sequence | Val 77 - Leu 233 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−DIF, TNF-alpha, TNFA, TNFSF2, cachexin, cachectin, TNFα
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
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