Tocilizumab Antibody

SKU: orb1297803

Description

Tocilizumab is a humanized monoclonal antibody that neutralizes the interleukin-6 receptor (IL-6R), inhibiting both classical and trans IL-6 signaling pathways. It is a critical research tool for studying inflammatory diseases like rheumatoid arthritis and has been investigated in clinical research for severe COVID-19 cytokine storm.

Research Area

Immunology & Inflammation

Images & Validation

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Key Properties

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CAS Number	375823-41-9
MW	148 kDa
Purity	98.00%
Target	Interleukin

Bioactivity

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In Vivo	METHODS: HARA-B cells were inoculated into the left ventricle of nude mouse hearts. Three weeks after inoculation, Tocilizumab (Anti-Human IL6R) was intravenously injected weekly for the next three weeks (1.0 mg/100 μL, once every two weeks for three weeks). RESULTS The volume of each metastatic lesion in the brains of mice injected with Tocilizumab (Anti-Human IL6R) was significantly smaller. Read more...
In Vitro	METHODS: Tocilizumab (Anti-Human IL6R) (0.001–10,000 μg/ml) was used to culture Ba/F3-gp130-hIL-6R or Ba/F3-gp130-mIL-6R cells for 2 days, and cell proliferation was observed. RESULTS Tocilizumab (Anti-Human IL6R) could inhibit cell proliferation, with an IC50 value of 13.5 ng/mL. Read more...
Cell Research	Objective: To validate that IL-6 signaling is essential for TAM (tumor-associated macrophages)-enhanced CSC (cancer stem cells) function. Concentrations: 5 μg/ml. Incubation Time: 72 h. Method: For co-culture experiments, 12-well Transwell chambers were used with 2×105 HepG2 or Hep3B cells seeded in the lower chamber one day before co-culture and either 5×105 or 1×106 TAMs added in the upper chamber. Tocilizumab or human IgG antibody were added in indicated experiments to selected wells. After 24 to 72 hours, HCC cells and CD14+ TAM cells were collected separately for analysis. Cells:HepG2 cell lines, TAMs isolated from HCCs (hepatocellular carcinoma) Read more...

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Tocilizumab, respiratory failure, rheumatoid, Inhibitor, Interleukin Related, Interleukin, IL-6R, inhibit, Neutralizing, arthritis, Anti-Human IL6R, Humanized Antibody, antibody, dyspnoea, COVID-19, coronavirus disease

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Gene Symbol

Interleukin

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