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TOM1L1/SRCASM Antibody
SKU: orb19785
Description
Research Area
Epigenetics
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, FC, IF, WB |
|---|---|
| Dilution range | ELISA: 1:64000, WB: 0.3-1 μg/ml |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Target | TOM1L1 / SRCASM |
| Molecular Weight | 53 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Disclaimer | For research use only |
Alternative Names
−anti TOM1L1 antibody, anti SRCASM antibody, anti target of myb1 (chicken)-like 1 antibody, anti Src activating and signaling molecule antibody, anti target of myb 1-like 1 antibody, anti target of myb1-like 1 antibody
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Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
1 ug/ml staining of A431 lysate 1 (A) + peptide (B), A431 lysate 2 (C) + peptide (D), HEK293 (E) + peptide (F), and HEL (G) + peptide (H) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.
1 µg/ml staining of Human Cerebellum lysate (35 µg protein in RIPA buffer). Detected by chemiluminecence.
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
TOM1L1/SRCASM Antibody (orb19785)
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