Topoisomerase II alpha Antibody / TOP2A

SKU: orb1151532

Description

Rabbit monoclonal antibody to Topoisomerase II alpha

Images & Validation

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Item 1 of 5

Tested Applications	IHC-P, WB
Dilution range	Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Reactivity	Human
Application Notes	Optimal dilution of the recombinant Topoisomerase II alpha antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG
Clone No.	TOP2A/6570R
Immunogen	A portion of amino acids 1352-1493 was used as the immunogen for the recombinant Topoisomerase II alpha antibody.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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IHC staining of FFPE human lymph node tissue with recombinant Topoisomerase II alpha antibody (clone TOP2A/6570R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with recombinant Topoisomerase II alpha antibody (clone TOP2A/6570R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Negative control: IHC staining of FFPE human brain tissue using recombinant Topoisomerase II alpha antibody (clone TOP2A/6570R) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human HeLa and Jurkat cell lysates using recombinant Topoisomerase II alpha antibody (clone TOP2A/6570R). Predicted molecular weight ~174 kDa.

SDS-PAGE analysis of purified, BSA-free recombinant Topoisomerase II alpha antibody (clone TOP2A/6570R) as confirmation of integrity and purity.

Quick Database Links

UniProt

P11388

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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