Recombinant TOP2A Antibody / Topoisomerase II alpha

SKU: orb1825133

Description

It recognizes a 170kDa protein, which is identified as topoisomerase IIAlpha. It shows no cross-reaction with Topoisomerase II beta or I. Topo II Alpha plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo II Alpha is also implicated in drug resistance of tumor cells and has been shown to be over-expressed in many human cancers. Decreased expression of Topo IIa is the predominant mechanism of resistance to several chemotherapeutic agents.

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Tested Applications	IHC-P
Dilution Range	Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 minutes at RT
Reactivity	Human
Application Notes	Optimal dilution of the recombinant TOP2A antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Recombinant
Isotype	Mouse IgG1, kappa
Clone No.	rTOP2A/8339
Immunogen	A recombinant human Topoisomerase II alpha protein fragment (aa1300-1500) was used as the immunogen for the recombinant TOP2A antibody.
Purification	Protein A/G affinity
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Recombinant TOP2A Antibody / Topoisomerase II alpha

IHC staining of FFPE human tonsil tissue with recombinant TOP2A antibody (clone rTOP2A/8339). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

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UniProt

P11388

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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