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Description
Research Area
Cancer Biology, Cardiovascular Research, Cell Biology, Immunology & Inflammation, Infectious Disease & Virology, Protein Biochemistry, Signal Transduction
Images & Validation
−Item 1 of 3
| Tested Applications | IF, WB |
|---|---|
| Dilution Range | IF - 1:10-50, WB - 1:1000 |
| Reactivity | Human |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1,K |
| Clone No. | B436EV38X5X6 |
| Immunogen | This TRAF2 monoclonal antibody is generated from mouse immunized with TRAF2 recombinant protein. |
| Target | TRAF2 {ECO:0000303|PubMed:28489822, ECO:0000312|HGNC:HGNC:12032} |
| Molecular Weight | 55859 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−TNF receptor-associated factor 2, 632-, E3 ubiquitin-protein ligase TRAF2, Tumor necrosis factor type 2 receptor-associated protein 3, TRAF2, TRAP3
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All lanes: Anti- at 1:1000 dilution. Lane 1: 293 whole cell lysate. Lane 2: Hela whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 56 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Confocal immunofluorescent analysis of TRAF2 Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
TRAF2/MB10188 antibody western blot analysis in T47D cell line lysates (35 μg/lane). This demonstrates the TRAF2/MB10188 antibody detected the TRAF2/MB10188 protein (arrow).
Quick Database Links
Gene Symbol
TRAF2 {ECO:0000303|PubMed:28489822, ECO:0000312|HGNC:HGNC:12032}
UniProt
RefSeq (Protein):NP_066961.2
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_066961.2 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
TRAF2 Antibody (orb1939374)
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