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TREM1 Rabbit Polyclonal Antibody
SKU: orb158644
Featured
Description
Images & Validation
−Item 1 of 4
| Tested Applications | FC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test |
| Reactivity | Human |
| Predicted Reactivity | Rat |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human TREM1 (151-234/234aa) |
| Target | TREM1 |
| Molecular Weight | 24 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Triggering receptor expressed on myeloid cells 1; TREM 1; TREM-1; Triggering receptor expressed on monocytes 1; Triggering receptor TREM 1; Triggering receptor TREM1; Triggering-receptor TREM 1; Triggering-receptor TREM-1; Triggering-receptor TREM1; TREM1_HUMAN; CD354.
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Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TREM1) Polyclonal Antibody, Unconjugated (orb158644) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Primary Antibody: Rabbit anti-TREM1 antibody, catalog number: orb158644, Isotype Control Antibody: Rabbit IgG, Secondary Antibody: F (ab')2 fragment goat anti-rabbit IgG-PE. Positive control: U937 (4% paraformaldehyde for 10 min at room temperature).
Primary Antibody: Rabbit anti-TREM1 antibody, catalog number: orb158644, Isotype Control Antibody: Rabbit IgG, Secondary Antibody: F (ab')2 fragment goat anti-rabbit IgG-PE. Positive control: U937 (4% paraformaldehyde for 10 min at room temperature).
Sample: hcclm3 (Human) Cell Lysate at 30 ug, K562 (Human) Cell Lysate at 30 ug, Primary: Anti-TREM1 (orb158644) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 24 kD, Observed band size: 21 kD.
Quick Database Links
Gene Symbol
TREM1
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IF
Immunofluorescence
TREM1 Rabbit Polyclonal Antibody (orb158644)
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