Queen's Award Received in 2021 ISO 9001 Certified Delivered over 1,000,000 bio-reagents to life science researchers Trusted by Life Science Communities
Cart summary

You have no items in your shopping cart.

TRIM29 Antibody

SKU: orb1249182

Description

TRIM29 Antibody

Research Area

Immunology & Inflammation

Images & Validation

Tested ApplicationsELISA, FC, IF, IHC, WB
ReactivityHuman
Predicted ReactivityBovine, Mouse, Rat
Application Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 37kDa band observed in Human Peripheral Blood Mononucleocytes (PBM) and in lysates of cell lines HeLa. An additional 70kDa band was seen in some PBM lysates (calculated MW of 37.3kDa according to NP_001317311.1 (isoform 2) and 65.8kDa according to NP_036233.2 (isoform 1). Recommended concentration: 0.1-1µg/mL. Primary incubation 1 hour at room tempetature.Immunohistochemistry:In paraffin embedded Human Skin shows staining of subnuclear structures and cytoplasm in keratinocytes. Recommended concentration: 3.75µg/mL.Immunofluorescence: Strong expression of the protein seen in the Cytoplasm and Intermediate filaments of A431 cells. Strong expression of the protein seen in the Nucleus of U2OS cells. Recommended concentration: 10µg/mL. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-EKDRIKSFTTNE
TargetTRIM29
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

TRIM29, tripartite motif-containing 29, ATDC, FLJ36085, ataxia-telangiectasia group D-associated protein, tripartite motif protein TRIM29

Similar Products

  • TRIM29/ATDC Antibody [orb20473]

    ELISA,  FC,  IF,  IHC,  WB

    Bovine, Mouse, Rat

    Human

    Polyclonal

    Unconjugated

    100 μg
  • TRIM29 Antibody [orb518589]

    ELISA,  IHC,  WB

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 50 μl
  • TRIM29 Antibody [orb388131]

    ELISA,  IHC

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg, 100 μg, 100 μg (without BSA and Azide)
  • TRIM29 antibody [orb2276287]

    IHC

    Human

    Mouse

    Monoclonal

    Unconjugated

    1 ml, 0.1 ml, 0.5 ml
  • TRIM29 Antibody [orb2309325]

    IHC

    Human

    Rabbit

    Monoclonal

    Unconjugated

    100 μg (without BSA and Azide), 20 μg, 100 μg
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

Select a size below

0.1 mg
$ 920.00
Free Secondary Antibody (20 ul)0/0

Please add an antibody product to your cart first.

DispatchUsually dispatched within 5-10 working days
Bulk Enquiry