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Description
Research Area
Epigenetics & Chromatin
Images & Validation
−
| Tested Applications | ChIP, DOT, IHC, WB |
|---|---|
| Dilution Range | WB1:500 - 1:2000, IHC-P: -P1:50 - 1:200, IF1:50 - 1:200IP1:50 - 1:200, ChIP1:20 - 1:100, ChIPseq1:20 - 1:100 |
| Reactivity | Human, Mouse, Other, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | A synthetic trimethylated peptide around K4 of human histone H3(NP_003520.1). |
| Molecular Weight | 17KDa |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH 7.2 |
| Buffer/Preservatives | Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH 7.2 |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−H3.4;H3/g;H3FT;H3t;HIST3H3;Histone H3;HIST1H3A
Similar Products
−TriMethyl-Histone H3-K4 Rabbit Polyclonal Antibody [orb3131264]
WB
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
50 μg, 100 μg
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Quick Database Links
UniProt
UniProt Details
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
DOT
Dot Blot
ChIP
Chromatin Immunoprecipitation
Available Sizes
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