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Trk A + B + C Recombinant Rabbit Monoclonal Antibody
SKU: orb612228
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Description
Images & Validation
−Item 1 of 12
| Tested Applications | ICC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB (Mouse, Rat)=1:500-2000, IHC-P (Human, Mouse, Rat)=1:50-200, IHC-F (Human, Mouse, Rat)=1:50-200, IF (Human, Mouse, Rat)=1:50-200, ICC/IF=1:50-200 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | 5T6 |
| Immunogen | KLH conjugated synthetic peptide derived from human Trk-B : 800-822/822 |
| Target | NTRK2 |
| Molecular Weight | Calculated molecular weight: 90kDa. Detected molecular weight: 130kDa. |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−DEE58; EIEE58; GP145-TrkB; OBHD; TRKB; trk-B; GP145-TrkB/GP95-TrkB; Tkrb; RATTRKB1; TRKB1; NTRK2_HUMAN; NTRK2; GP145-TrkB (Trk-B); Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; Tropomyosin-related kinase B; 2.7.10.1; NTRK2_MOUSE; GP145-TrkB/GP95-TrkB (Trk-B); NTRK2_RAT; TrkB tyrosine kinase (Trk-B);
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25 ug total protein per lane of various lysates probed with Trk A + B + C Recombinant Rabbit Monoclonal Antibody (orb612228) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum. Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min. The section was incubated with Trk A + B + C Recombinant Rabbit Monoclonal Antibody (orb612228) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L, HRP conjugated (orb572747) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum. Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min. The section was incubated with Trk A + B + C Recombinant Rabbit Monoclonal Antibody (orb612228) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L, HRP conjugated (orb572747) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum. Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min. The section was incubated with Trk A + B + C Recombinant Rabbit Monoclonal Antibody (orb612228) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L, HRP conjugated (orb572747) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum. Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min. The section was incubated with Trk A + B + C Recombinant Rabbit Monoclonal Antibody (orb612228) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L, HRP conjugated (orb572747) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum. Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min. The section was incubated with Trk A + B + C Recombinant Rabbit Monoclonal Antibody (orb612228) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L, HRP conjugated (orb572747) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Glioma. Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min. The section was incubated with Trk A + B + C Recombinant Rabbit Monoclonal Antibody (orb612228) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L, HRP conjugated (orb572747) and DAB staining.
(Negative control) Paraformaldehyde-fixed, paraffin embedded Rat Kidney. Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min. The section was incubated with Trk A + B + C Recombinant Rabbit Monoclonal Antibody (orb612228) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L, HRP conjugated (orb572747) and DAB staining.
(Negative control) Paraformaldehyde-fixed, paraffin embedded Mouse Kidney. Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min. The section was incubated with Trk A + B + C Recombinant Rabbit Monoclonal Antibody (orb612228) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L, HRP conjugated (orb572747) and DAB staining.
(Negative control) Paraformaldehyde-fixed, paraffin embedded Human Kidney. Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min. The section was incubated with Trk A + B + C Recombinant Rabbit Monoclonal Antibody (orb612228) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L, HRP conjugated (orb572747) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum. Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min. The section was incubated with Trk A + B + C Recombinant Rabbit Monoclonal Antibody (orb612228) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG H&L, BF594 conjugated (orb1809473), DAPI was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum. Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min. The section was incubated with Trk A + B + C Recombinant Rabbit Monoclonal Antibody (orb612228) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG H&L, BF594 conjugated (orb1809473), DAPI was used to stain the cell nuclei.
Quick Database Links
Gene Symbol
NTRK2
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
ICC
Immunocytochemistry
Trk A + B + C Recombinant Rabbit Monoclonal Antibody (orb612228)
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