TROP2 Antibody / TACSTD2

SKU: orb2641251

Description

This intronless gene encodes a carcinoma-associated antigen defined by the monoclonal antibody GA733. This antigen is a member of a family including at least two type I membrane proteins. It transduces an intracellular calcium signal and acts as a cell surface receptor. [Wiki]

Images & Validation

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Tested Applications	IF, IHC-P
Dilution range	Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Reactivity	Human
Application Notes	The stated application concentrations are suggested starting points. Titration of the TROP2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1, kappa
Clone No.	TCSR2-1
Immunogen	A portion of amino acids 31-274 were used as the immunogen for the TROP2 antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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IHC testing of FFPE human colon carcinoma with TROP2 antibody (clone TCSR2-1). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Immunofluorescent staining of human MCF7 cells with TROP2 antibody (green) and Reddot nuclear stain (red).

Quick Database Links

UniProt

P09758

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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Immunofluorescence

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