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KO/KD
Validated
Validated
Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 4
| Tested Applications | KO/KD Validated, WB |
|---|---|
| Reactivity | Mouse |
| Predicted Reactivity | Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Immunogen | The immunogen is a synthetic peptide corresponding to a region of Mouse |
| Target | Trp53 |
| Protein Sequence | Synthetic peptide located within the following region: EEFFEGPSEALRVSGAPAAQDPVTETPGPVAPAPATPWPLSSFVPSQKTY |
| Molecular Weight | 43kDa |
| Purification | Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Concentration | 0.5 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−anti BCC7 antibody, anti FLJ92943 antibody, anti HGNC11998 antibody, anti LFS1 antibody, anti p53 antibody, anti p53 Cellular Tumor Antigen antibody, anti p53 tumor suppressor antibody, anti Phosphoprotein p53 antibody, anti Tp53 antibody, anti TRP53 antibody, anti Tumor protein 53 antibody, anti Tumor protein p53 antibody, anti TP53 antibody
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Sample Tissue: Mouse Pancreas, Antibody dilution: 1 ug/ml.
Sample Tissue: Mouse Pancreas, Antibody dilution: 1 ug/ml.
WB Suggested Anti-TRP53 Antibody, Positive Control: Lane 1: 40 ug C57/B6 control mouse G.I, Lane 2: 40 ug C57/B6 mouse G.I. treated with Irinotecan, Lane 3: 40 ug p53 KO HCT-116 cells Lane 4: 40 ug C57/B6 mouse treated with 15 Gy ionizing radiation, Primary Antibody Dilution: 1:1000, Secondary Antibody: Goat anti-rabbit-HRP, Secondry Antibody Dilution: 1:2500.
WB Suggested Anti-Trp53 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:312500, Positive Control: SP2/0 cell lysate.
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Trp53 Rabbit Polyclonal Antibody (orb333708)
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