TRPM7 Rabbit Polyclonal Antibody

SKU: orb1960

Description

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, FC
Dilution range	Flow-Cyt=1ug/Test, ELISA=1:5000-10000
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Equine, Gallus, Mouse, Porcine, Rabbit, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human TRPM7 (801-900/1865aa)
Target	TRPM7
Molecular Weight	180, 205 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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CHAK 1; CHAK; CHAK1; Channel kinase 1; Channel-kinase 1; FLJ20117; FLJ25718; homolog of mouse transient receptor potential-phospholipase C-interacting kinase; Long transient receptor potential channel 7; LTRPC 7; LTRPC ion channel family member 7; LTrpC-7; LTRPC7; transient receptor potential cation channel subfamily M member 7; TRP PLIK; TRP-PLIK; TRPM 7; TRPM7; TRPM7_HUMAN.

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Blank control (black line): MCF-7. Primary Antibody (green line): Rabbit Anti-TRPM7 antibody (orb1960), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

TRPM7

UniProt

Q96QT4

UniProt Details

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No UniProt data available

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Protocol Information

Flow Cytometry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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