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Tubastatin A

SKU: orb1306944

Description

Tubastatin A is a potent and highly selective HDAC6 inhibitor, exhibiting an IC50 of 15 nM in biochemical assays with >1000-fold selectivity over other HDAC isoforms except HDAC8. It is widely used in both in vitro and in vivo research to investigate HDAC6's role in oncology, neurology, and immunology, including studies on neurodegenerative disease models and cancer cell proliferation.
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Research Area

Cell Biology, Epigenetics & Chromatin, Infectious Disease & Virology, Molecular Biology

Images & Validation

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Tubastatin A

Key Properties

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CAS Number1252003-15-8
MW335.4
Purity98.36%
FormulaC20H21N3O2
SMILESCN1CCc2c(C1)c1ccccc1n2Cc1ccc(cc1)C(=O)NO
TargetHDAC,Apoptosis,Autophagy,Antibacterial
SolubilityEthanol:< 1 mg/mL (insoluble or slightly soluble);DMSO:35 mg/mL (104.35 mM);H2O:< 1 mg/mL (insoluble or slightly soluble);10% DMSO+40% PEG300+5% Tween 80+45% Saline:1 mg/mL (2.98 mM)

Bioactivity

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Target IC50
HDAC8:854 nM|HDAC6:15 nM|HDAC1:16400 nM
In Vivo
Tubastatin A (5 μM) dose-dependently protected neuronal cells from homocysteine-induced death, which was completely avoided at 10 μM. Tubastatin A (10 μM) slightly induced histone acetylation. When applied to cholangiocarcinoma cell lines, Tubastatin A (10 μM) induced an increase in the level of acetylated α-tubulin and the restoration of primary cilia expression, which correlated with the down-regulation of Hedgehog and MAPK signaling pathways, and a decrease in the rate of cell proliferation (50% on average) and infiltration (40%). Tubastatin A significantly inhibited TNF-α and IL-6 when acting on lipopolysaccharide-stimulated human THP-1 macrophages (IC50: 272/712 nM). When acting on mouse Raw 264.7 macrophages, Tubastatin A dose-dependently inhibited nitric oxide secretion (IC50: 4.2 μM).
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In Vitro
Tubastatin A (5 μM) dose-dependently protected neuronal cells from homocysteine-induced death, which was completely avoided at 10 μM. Tubastatin A (10 μM) slightly induced histone acetylation. When applied to cholangiocarcinoma cell lines, Tubastatin A (10 μM) induced an increase in the level of acetylated α-tubulin and the restoration of primary cilia expression, which correlated with the down-regulation of Hedgehog and MAPK signaling pathways, and a decrease in the rate of cell proliferation (50% on average) and infiltration (40%). Tubastatin A significantly inhibited TNF-α and IL-6 when acting on lipopolysaccharide-stimulated human THP-1 macrophages (IC50: 272/712 nM). When acting on mouse Raw 264.7 macrophages, Tubastatin A dose-dependently inhibited nitric oxide secretion (IC50: 4.2 μM).
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Cell Research
Anchorage-independent growth is assessed by growing cells in soft agar. About 25,000 cells suspended in 0.4% agar in culture media are layered over a 1% agar layer in a 6-well plate. Media are added twice a week and pictures are taken after 21 days of incubation. The number and size of colonies are analyzed using the Gel-Pro software.(Only for Reference)
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Storage & Handling

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StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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Tubastatin A, Tubastatin A BASE, Apoptosis, Autophagy, HDAC, HDAC6, Histone deacetylases

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Compound Identifiers

PubChem CID
49850262 ↗

Key Properties

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No computed properties available.

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Tubastatin A (orb1306944)

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% DMSO +
%+
% Tween 80 +
%

Available Sizes

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1 ml x 10 mM (in DMSO)
$ 80.00
10 mg
$ 90.00
25 mg
$ 100.00
50 mg
$ 120.00
100 mg
$ 160.00
200 mg
$ 220.00
500 mg
$ 330.00
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