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Validated
Description
Research Area
Images & Validation
−| Tested Applications | KO/KD Validated, WB |
|---|---|
| Dilution Range | WB 1:1000~2000 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | BQVK7J4 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1-150 of human TUBB3 (NP_006077) produced in E.coli. |
| Target | TUBB3 |
| Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose) |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Western blot analysis of Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and TUBB3-Knockout Hela cells (KO) were separated by SDS-PAGE and immunoblotted using beta III Tubulin (TUBB3) antibody
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TUBB3 Antibody (orb1338671)
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