Tumor Necrosis Factor beta

SKU: orb511126

Description

Tumor Necrosis Factor – Beta (TNF-β), also known as lymphotoxin-alpha is a human recombinant protein that can be used in a variety of applications including cytotoxicity, cell proliferation assays, and apoptosis. This product is best used for inducing apoptosis to create a positive control.

Images & Validation

−

Application Notes

1. To make a 5 mg/mL DAPI stock solution (14.3 mM), dissolve the contents of one vial (10 mg) in 2 mL of deionized water or dimethylformamide (DMF). Note: DAPI is not very soluble in phosphate-buffered saline (PBS)., 2. For long term storage, the stock solution can be aliquoted and stored at -20°C. For short term storage, the solution can be kept at 2-6°C, protected from light. When handled properly, DAPI solutions are stable for at least six months., 3. Dilute the DAPI stock solution to 3 µM in staining buffer. A 1 mL volume will be required for each cell sample., 4. Centrifuge the cell suspension and discard the supernatant. Tap to loosen the pellet and add 1 mL of DAPI diluted in staining buffer., 5. Incubate for 15 minutes at room temperature., 6. Analyze by flow cytometry in the presence of the dye. If the cells are to be viewed by fluorescence microscopy, centrifuge the sample, remove the supernatant and resuspend cells in fresh buffer. Apply a drop of the suspension to a microscope slide, cover with a coverslip and view., For sample preparation protocols for adherent cells and suspension cells, please see the DAPI product datasheet.

Key Properties

−

Storage & Handling

−

Storage	-20°C
Disclaimer	For research use only

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Quick Database Links

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Find More Protocols