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TWIST1 Antibody
Description
Images & Validation
−| Tested Applications | FC, ICC, IHC, WB |
|---|---|
| Dilution range | WB: 1/500 - 1/2000; IHC: 1/100 - 1/500; ICC: 1/50 - 1/500; FCM: 1/200 - 1/400; ELISA: 1/10000 |
| Reactivity | Human, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 10E4E6 |
| Immunogen | Purified recombinant fragment of human TWIST1 (AA: 9-74) expressed in E. Coli. |
| Target | TWIST1 |
| Molecular Weight | 21kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide |
| Concentration | Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−TWIST Rabbit Polyclonal Antibody [orb13736]
IF, IHC-Fr, IHC-P
Human
Mouse, Rat
Rabbit
Polyclonal
Unconjugated
200 μl, 50 μl, 100 μlTWIST1 Rabbit Polyclonal Antibody [orb329955]
IHC, WB
Bovine, Canine, Equine, Guinea pig, Mouse, Porcine, Rabbit, Rat, Zebrafish
Human
Rabbit
Polyclonal
Unconjugated
100 μl

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Western blot analysis of human TWIST1 using TWIST1 antibody

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of TWIST1 antibody

Flow cytometric analysis of Hela cells lysates using TWIST1 antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded cervical cancer tissues using TWIST1 antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded colon cancer tissues using TWIST1 antibody

Western blot analysis of HEK293 (Lane 1) HEK293 (Lane 2) cell lysate using TWIST1 antibody

Western blot analysis of NIH/3T3 (Lane 1), JURKAT (Lane 2), HELA (Lane 3), A549 (Lane 4), RAJI (Lane 5) OCM-1 (Lane 6) cell lysate using TWIST1 antibody
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Protocol Information
TWIST1 Antibody (orb153461)
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