UBE2M Rabbit Polyclonal Antibody

SKU: orb578688

Description

Rabbit polyclonal antibody to UBE2M

Research Area

Protein Biochemistry

Images & Validation

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Item 1 of 6

Tested Applications	IHC, WB
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Canine, Equine, Guinea pig, Rabbit, Rat

Related Conjugates & Formulations

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Biotin

FITC

HRP

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	The immunogen is a synthetic peptide directed towards the N terminal region of human UBE2M
Target	UBE2M
Protein Sequence	Synthetic peptide located within the following region: IKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDINELNLPKTCDISF
Molecular Weight	21kDa
Purification	Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration	0.5 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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UBC12, hUbc12, UBC-RS2

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Sample Type: 2. mouse brain extracts (80 ug), Primary Antibody Dilution: 2 ug/ml, Secondary Antibody: IRDye 800CW goat anti-rabbit, Secondary Antibody Dilution: 1:20000.

Sample Type: NT2 cells, Red: Antibody, Blue: DAPI, Primary Dilution: 1 ug/50 ul antibody, Secondary Antibody: Alexa goat anti-rabbit 594.

Sample Type: Human 721_B, Antibody Dilution: 1.0 ug/ml. UBE2M is supported by BioGPS gene expression data to be expressed in 721_B.

Sample Type: Human MCF7, Antibody Dilution: 1.0 ug/ml. UBE2M is supported by BioGPS gene expression data to be expressed in MCF7.

Sample Type: Human brain stem cells, Primary Antibody Dilution: 1:500, Secondary Antibody: Goat anti-rabbit Alexa-Fluor 594, Secondary Antibody Dilution: 1:1000, Color/Signal Descriptions: UBE2M: Red DAPI:Blue, Gene Name: UBE2M.

WB Suggested Anti-UBE2M Antibody Titration: 0.2-1 ug/ml, Positive Control: 721_B cell lysate. UBE2M is supported by BioGPS gene expression data to be expressed in 721_B.

Quick Database Links

Gene Symbol

UBE2M

UniProt

P61081

RefSeq (Protein):NP_003960

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_003960

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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