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Ubiquitin Antibody

SKU: orb606656

Description

Ubiquitin is a highly conserved and plays an essential role in the ubiquitin-proteasome pathway. In ubiquitination process, it is first activated by forming a thiol-ester complex with the activation component E1, which is then transferred to ubiquitin-carrier protein E2, followed by to ubiquitin ligase E3 for final delivery to epsilon-NH2 of the target protein lysine residue. IkB, p53, cdc25A, Bcl-2 etc. are shown as targets of ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis. Moreover, ubiquitin have been reported to bind covalently with pathological inclusions which are resistant to degradation e.g. neurofibrillary tangles/paired helical filaments in Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease etc.

Research Area

Epigenetics, NF-kB, Neuroscience, Signaling Pathways

Images & Validation

Tested ApplicationsFACS, IF, IHC-P, WB
Dilution RangeFlow cytometry: 1-2ug/10^6 cells,Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
ReactivityHuman
Application Notes
Optimal dilution of the Ubiquitin antibody should be determined by the researcher.

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeMouse IgG2c, kappa
Clone No.UBB/1748
ImmunogenA portion of amino acids 1-119 from the human protein was used as the immunogen for the Ubiquitin antibody.
PurificationProtein G affinity chromatography
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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Ubiquitin Antibody

Western blot testing of human Raji cell lysate with Ubiquitin antibody (clone UBB/1748). Expected molecular weight ~9 kDa.

Ubiquitin Antibody

IHC staining of FFPE human brain with Ubiquitin antibody (clone UBB/1748). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

Ubiquitin Antibody

IHC staining of FFPE human brain with Ubiquitin antibody (clone UBB/1748). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

Ubiquitin Antibody

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using Ubiquitin antibody (clone UBB/1748). These results demonstrate the foremost specificity of the UBB/1748 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Ubiquitin Antibody

SDS-PAGE analysis of purified, BSA-free Ubiquitin antibody (clone UBB/1748) as confirmation of integrity and purity.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
View Protocol
IF
Immunofluorescence
View Protocol

Ubiquitin Antibody (orb606656)

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20 μg
$ 370.00
100 μg
$ 750.00
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