UGT1A7 Rabbit Polyclonal Antibody

SKU: orb313418

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Description

Images & Validation

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Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Mouse, Rat
Predicted Reactivity	Porcine

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from rat UGT1A7 (431-531/531aa)
Target	UGT1A7
Molecular Weight	57 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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GNT1; UGT1A7; OTTHUMP00000065199; UD17_HUMAN; UDP glucuronosyltransferase 1A7; UDP glucuronosyltransferase; UDP glycosyltransferase 1 family polypeptide A7; UDP-glucuronosyltransferase 1 family polypeptide A7s; UDP-glucuronosyltransferase 1-7; UDP-glucuronosyltransferase 1-G; UDP-glucuronosyltransferase 1A7; UDPGT; UDPGT 1-7; UGT-1G; UGT1; UGT1*7; UGT1-07; UGT1.7; UGT1A7; UGT1G.

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Paraformaldehyde-fixed, paraffin embedded (Rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (UDP- glycosyltransferase 1 family polypeptide A7, UTG1A7) Polyclonal Antibody, Unconjugated (orb313418) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Sample: Kidney (Mouse) Lysate at 40 ug, Primary: Anti-UGT1A7 (orb313418) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 57kD, Observed band size: 57kD.

Sample: Liver (Mouse) Lysate at 40 ug, Primary: Anti-UGT1A7 (orb313418) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 57kD, Observed band size: 57kD.

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Gene Symbol

UGT1A7

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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