UVRAG Antibody (Biotin)

SKU: orb377189

Description

Rabbit polyclonal antibody against UVRAG conjugated to Biotin.

Images & Validation

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Tested Applications	ICC, IF, WB
Dilution range	WB (1:1000); ICC/IF (1:100)
Reactivity	Human, Mouse, Rat
Application Notes	A 1:1000 dilution of SPC-606 was sufficient for detection of UVRAG on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide from the mid-protein of human UVRAG
Target	UVRAG
Molecular Weight	78.1kDa
Purification	Peptide Affinity Purified
Conjugation	Biotin

Storage & Handling

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Storage	Conjugated antibodies should be stored according to the product label
Buffer/Preservatives	136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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UVRAG, UV radiation resistance-associated, UVRAG_HUMAN, DHTX

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Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-UVRAG Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-UVRAG Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Late Endosome, Lysosome, Early Endosome. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) UVRAG Antibody (D) Composite.

Western blot analysis of Human HeLa and HEK293T cell lysates showing detection of ~100kDa UVRAG protein using Rabbit Anti-UVRAG Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human HeLa (20 μg). Lane 3: Human 293T (20 μg). Load: 20 μg. Block: 5% Skim Milk for 1 hour at RT. Primary Antibody: Rabbit Anti-UVRAG Polyclonal Antibody at 1:200 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~100kDa.

Western blot analysis of Rat Liver showing detection of ~100kDa UVRAG protein using Rabbit Anti-UVRAG Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Rat Liver (20 μg). Load: 20 μg. Block: 5% Skim Milk for 1 hour at RT. Primary Antibody: Rabbit Anti-UVRAG Polyclonal Antibody at 1:200 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~100kDa.

Quick Database Links

Gene Symbol

UVRAG

UniProt

Q9P2Y5

NCBI Gene ID

7405

RefSeq (Protein):NP_003360.2

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_003360.2

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Protocol Information

Western Blot (IB, immunoblot)

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Immunofluorescence

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ICC

Immunocytochemistry

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