Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG
Clone No.	5D7
Immunogen	KLH conjugated V5-tag
Purification	Affinity purified by Protein G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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V5-tag; GKPIPNPLLGLDST epitope tag; GKPIPNPLLGLDST tag; Protein Rev; Regulator of expression of viral proteins; rev; V5 epitope tag.

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Sample: Lane 1: V5-Tagged Fusion Protein Overexpression E.coli Lysate at 2 ug Lane 2: V5-Tagged Fusion Protein Overexpression E.coli Lysate at 2 ug Primary: Anti-V5 tag (orb500328) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 45 kD.

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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