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VAChT Antibody
SKU: orb67481
Featured
Description
Research Area
Disease Biomarkers, Neuroscience, Pharmacology & Drug Discovery
Images & Validation
−Item 1 of 3
| Tested Applications | ICC, IF, IHC, WB |
|---|---|
| Dilution Range | WB (1:1000), IHC-P (1:200), ICC/IF (1:100) |
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Recombinant Antibody |
|---|---|
| Host | Mouse |
| Clonality | Recombinant |
| Isotype | IgG1 |
| Clone No. | S6 |
| Immunogen | Synthetic peptide amino acids 521-532 of human VAChT |
| Target | VAChT |
| Molecular Weight | 56kDa |
| Purification | Protein A Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS pH 7.4, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Vesicular Acetylcholine Transporter, VAChT, SLC18A3, Slc18a3, Solute carrier family 18 member 3, Solute carrier family 18 (vesicular acetylcholine) member 3, Solute carrier family 18 (vesicular monoamine) member 3, Vesamicol-binding protein, MGC12716, rVAT, Unc-17
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Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VAChT Monoclonal Antibody, Clone N6/38. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VAChT Monoclonal Antibody at 1:200 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VAChT Antibody (D) Composite.
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VAChT Monoclonal Antibody, Clone N6/38. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VAChT Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) VAChT Antibody. (D) Composite.
Western Blot analysis of Rat brain membrane lysate showing detection of VAChT protein using Mouse Anti-VAChT Monoclonal Antibody, Clone N6/38. Primary Antibody: Mouse Anti-VAChT Monoclonal Antibody at 1:1000.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Gene Details
− No NCBI Gene data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_003046.2 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ICC
Immunocytochemistry
VAChT Antibody (orb67481)
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