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Featured
KO/KD
Validated
Validated
Description
Research Area
Cancer Biology, Cell Biology, Neuroscience, Signal Transduction
Images & Validation
−Item 1 of 5
| Tested Applications | IF, IHC, KO/KD Validated, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-101 of human Vimentin (NP_003371.2). |
| Target | VIM |
| Molecular Weight | Observed: 57kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Vimentin, VIM
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Western blot analysis of extracts of various cell lines, using Vimentin antibody (orb1274528) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) 293T cells, using Vimentin antibody (orb1274528) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human tonsil using Vimentin antibody (orb1274528) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using Vimentin antibody (orb1274528) at dilution of 1:150 (40x lens).
Immunofluorescence analysis of U2OS cells using Vimentin antibody (orb1274528) at dilution of 1:100. Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
VIM
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
VIM Antibody (orb1274528)
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