Vinculin Recombinant Rabbit Monoclonal Antibody (Loading Control)

SKU: orb704245

Description

Research Area

Actin Binding Proteins, Actina, CARdiovascular Research, Cell Biology

Images & Validation

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Item 1 of 1

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Rat

Related Conjugates & Formulations

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HRP

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	5G7
Immunogen	Recombinant Human Vinculin
Target	VCL
Molecular Weight	124 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CMD1W; CMH15; Epididymis luminal protein 114; HEL114; Metavinculin; MV; MVCL; OTTHUMP00000019861; OTTHUMP00000019862; VCL; VINC; VINC_HUMAN.

Quality Guarantee

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Vinculin Recombinant Rabbit Monoclonal Antibody (Loading Control)

Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Human HeLa cell lysates, Lane 3: Human U251 cell lysates, Lane 4: Human K562 cell lysates, Lane 5: Human HUVEC cell lysates, Primary: Anti-Vinculin (Loading Control) (orb704245) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 124 kDa, Observed band size: 135 kDa.