von Willebrand Factor Antibody

SKU: orb385836

Description

von Willebrand Factor (vWF) is a glycoprotein produced by bone marrow cells and endothelial cells lining the inside surface of blood vessels. Its primary function is platelet adhesion, binding to Factor VIII, collagen and platelets, to coagulate blood at the site of wounding. The enzyme VWFCP, or vWF-cleaving protease, facilitates clotting by cutting the protein into subunits, increasing its binding capacity.Deficiency or dysfunction of the protein increases the tendency of wounds to bleed, or to bleed more. ï¿½Over 300 gene mutations have been identified and classified into three types. ï¿½Type 1 von Willebrand Factor disease is characterized by reduced amounts in the bloodstream, Type 2 by reduced binding ability and Type 3 by a nonfunctional protein.

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Tested Applications	IHC-P
Dilution Range	Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Reactivity	Human
Application Notes	Variations in protocols, secondaries and substrates may require the von Willebrand Factor antibody to be titered for optimal performance.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1, kappa
Clone No.	WFA52-2
Immunogen	A recombinant human protein fragment was used as the immunogen for this von Willebrand Factor antibody.
Purification	Protein G purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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IHC testing of FFPE human pancreas and von Willebrand Factor antibody (clone WFA52-2). Required HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min.

IHC staining of human bone marrow with von Willebrand Factor antibody (clone WFA52-2). Required HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min.

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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