B7-H4 Antibody / VTCN1

SKU: orb2643948

Description

T cell activation and immune function are regulated by the innate immune system through positive and negative costimulatory proteins. One such protein, B7-H4 (B7-homolog 4), belongs to the B7 immunoglobulin superfamily of ligand-lymphocyte interacting proteins. Expressed primarily on the membrane of lymphoid cells, B7-H4 is an immuno-inhibitory protein that interacts with receptors on the surface of T lymphocytes, thus mediating cellular and humoral immune responses. Overexpression of the B7-H4 protein is associated with certain malignancies, including ovarian and breast cancer, as its interaction with T cells suppresses tumor-associated immunity. Current research suggests that, similar to Mucin 16 (CA-125), B7-H4 may be a useful biomarker for the early detection of ovarian cancer.

Images & Validation

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Tested Applications	ELISA, FACS, IF, IHC-P, WB
Dilution range	ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/10^6 cells in 0.1ml,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Reactivity	Human
Application Notes	Optimal dilution of the B7-H4 antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG2a, kappa
Clone No.	B7H4/1788
Immunogen	A recombinant human partial protein was used as the immunogen for this B7-H4 antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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Immunofluorescent staining of fixed human SK-BR-3 cells with B7-H4 antibody (clone B7H4/1788, green) and Reddot nuclear stain (red).

Flow cytometry testing of fixed human SK-BR-3 cells with B7-H4 antibody (clone B7H4/1788); Red=isotype control, Blue= B7-H4 antibody.

IHC staining of FFPE human testicular carcinoma with B7-H4 antibody (clone B7H4/1788). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human HCT-116 cell lysate with B7-H4 antibody. Predicted molecular weight: ~31 kDa.

SDS-PAGE analysis of purified, BSA-free B7-H4 antibody (clone B7H4/1788) as confirmation of integrity and purity.

Quick Database Links

UniProt

Q7Z7D3

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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