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VWF Recombinant Rabbit Monoclonal Antibody
SKU: orb1599573
Featured
Description
Research Area
Angiogenesis, Cancer Research, Cardiovascular Research, Cardiovascuolar, Cell Markers, cardiovascular Research
Images & Validation
−Item 1 of 2
| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | 9C2 |
| Immunogen | Recombinant human VWF protein |
| Target | VWF |
| Molecular Weight | 309 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 1*TBS (pH7.4), 0.05% rAlbumin, 40% Glycerol. Preservative: 0.02% Proclin300. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Von Willebrand Factor; Coagulation factor VIII; F8VWF; Factor VIII related antigen; von Willebrand antigen 2; Von Willebrand antigen II; Von Willebrand disease; VWD; VWF_HUMAN
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Von Willebrand Factor antibody (orb1599573) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb1599573) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of Von Willebrand Factor on human lung lysates with Rabbit anti-Von Willebrand Factor antibody (orb1599573) at 1/1000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 309 kDa, Observed band size: 250 kDa, Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (orb1599573) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300000 dilution was used for 1 hour at room temperature.
Quick Database Links
Gene Symbol
VWF
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
VWF Recombinant Rabbit Monoclonal Antibody (orb1599573)
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