You have no items in your shopping cart.
Wnt5a Antibody
SKU: orb414035
Description
Research Area
Cancer Biology, Neuroscience, Signal Transduction, Stem Cell & Developmental Biology
Images & Validation
−Item 1 of 2
| Tested Applications | IHC, WB |
|---|---|
| Dilution range | WB (1:1000); IHC (1:50) |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide of Human Wnt5a (200-300 aa), conjugated to Keyhole Limpet Haemocyanin (KLH). |
| Target | Wnt5a |
| Molecular Weight | 42.3 kDa, 35 kDa |
| Purification | Peptide Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−WNT5A, Wnt Family Member 5A, Wingless-Type MMTV Integration Site Family Member 5A, WNT-5A, Protein Wnt-5a, HWNT5A, Epididymis Secretory Sperm Binding Protein
Similar Products
−- Item 1 of 6
WNT5A Rabbit Polyclonal Antibody [orb13762]
FC, IF, IHC-Fr, IHC-P, WB
Bovine, Porcine, Rabbit
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
50 μl, 100 μl, 200 μl - Item 1 of 6
WNT5A Antibody (Center) [orb1930406]
FC, IHC-P, WB
Rabbit
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
200 μl - Item 1 of 7
WNT5A Rabbit Polyclonal Antibody [orb574059]
IHC, WB
Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat, Zebrafish
Human
Rabbit
Polyclonal
Unconjugated
100 μl - Item 1 of 1
Human Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) ELISA Kit [orb779389]
Human
0.16-10 ng/mL
0.056 ng/mL
96 T, 48 T - Item 1 of 1
Rat Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) ELISA Kit [orb781565]
Rat
0.16-10 ng/mL
0.063 ng/mL
96 T, 48 T
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.
Immunohistochemistry analysis using Rabbit Anti-Wnt5a Polyclonal Antibody. Tissue: Thyroid Carcinoma. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-Wnt5a Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.
Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~42.3 kDa Wnt5a protein using Rabbit Anti-Wnt5a Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Wnt5a Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~42.3 kDa. Other Band (s): ~35 kDa isoform 2.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Gene Details
− No NCBI Gene data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001243034.1 |
|---|
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
Wnt5a Antibody (orb414035)
- 0.0
Based on 0 reviews
Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.
Login to Submit a ReviewAvailable Sizes
Select a size below
Usually dispatched within 2-3 weeks