Recombinant WT1 Antibody / Rabbit Monoclonal

SKU: orb750181

Description

Wilms tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilms tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.

Research Area

Cancer, Epigenetics

Images & Validation

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Tested Applications	IHC-P
Dilution Range	Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Reactivity	Human, Rat
Application Notes	Optimal dilution of the recombinant WT1 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	WT1/1434R
Immunogen	A recombinant protein was used as the immunogen for the recombinant WT1 antibody.
Purification	Protein A affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Recombinant WT1 Antibody / Rabbit Monoclonal

IHC testing of FFPE human mesothelioma tissue with recombinant WT1 antibody (clone WT1/1434R). HIER: steam sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min.

IHC testing of FFPE rat testis tissue with recombinant WT1 antibody (clone WT1/1434R). HIER: steam sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min.

SDS-PAGE analysis of purified, BSA-free recombinant WT1 antibody (clone WT1/1434R) as confirmation of integrity and purity.

Quick Database Links

UniProt

P19544

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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