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XRCC5 Antibody (Center K439)
SKU: orb1937835
Description
Images & Validation
−Item 1 of 4
| Tested Applications | FC, IF, WB |
|---|---|
| Dilution Range | WB - 1:1000, IF - 1:10-50, FC - 1:10-50 |
| Reactivity | Human |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 82705 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−G22P2
Quality Guarantee
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Confocal immunofluorescent analysis of XRCC5 Antibody (Center K439) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
XRCC5 Antibody (Center K439) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
XRCC5 Antibody (CenterK439) western blot analysis in human placenta tissue lysates (35 ug/lane). This demonstrates the XRCC5 antibody detected the XRCC5 protein (arrow).
All lanes: Anti-XRCC5Antibody (CenterK439) at 1:1000 dilution. Lane 1: A549 whole cell lysate. Lane 2: Hela whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 83 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IF
Immunofluorescence
XRCC5 Antibody (Center K439) (orb1937835)
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