ZEB1 Rabbit Polyclonal Antibody

SKU: orb1294383

Description

Rabbit polyclonal antibody to ZEB1

Images & Validation

−

Item 1 of 3

Tested Applications	IF, WB
Dilution Range	Western Blot 1:500, Immuno fluorescence 1:200-1:300
Reactivity	Human, Mouse, Rat

Key Properties

−

Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit-IgG
Immunogen	Synthetic peptide / encompassing a sequence within the center region
Target	ZEB1
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Disclaimer	For research use only

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

All lanes: Anti-ZEB1 antibody at 1/500 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294383 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294383 anti-ZEB1 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Quick Database Links

Gene Symbol

ZEB1

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

Immunofluorescence

View Protocol

Find More Protocols