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Zebrafish Luteinizing Hormone (LH) ELISA Kit

SKU: orb782689
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Description

The Zebrafish Luteinizing Hormone (LH) ELISA Kit uses a competitive inhibition enzyme immunoassay technique to quantify Luteinizing Hormone (LH) in zebrafish serum, plasma and other biological fluids. The kit has a detection range of 468.75-30000 pg/mL and a sensitivity of 145.2 pg/mL.

Research Area

Metabolism Research

Images & Validation

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Tested ApplicationsELISA
Application Notes
standard: 100 mIU/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Zebrafish LH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish LH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Zebrafish LH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish LH in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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ReactivityZebrafish
Sample Typesserum, plasma and other biological fluids
Assay TypeSandwich
Assay Time3.5h
Range468.75-30000 pg/mL
Sensitivity145.2 pg/mL
Concentration100 mIU/mL

Storage & Handling

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StorageRefer to the Storage Guidelines in the Manual
Expiration DatePlease enquire.
DisclaimerFor research use only

Alternative Names

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ICSH ELISA Kit, Lutropin ELISA Kit, Interstitial Cell Stimulating Hormone ELISA Kit

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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Available Sizes

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48 T
$ 330.00
96 T
$ 470.00
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