Acetylated Lysine Antibody

SKU: orb1822476

Description

Mouse Anti-Acetylated Lysine Monoclonal IgG1

Research Area

Protein Biochemistry, Signal Transduction

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, ICC, IF, IHC, IP, WB
Dilution range	WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.
Reactivity	All
Application Notes	1 µg of SMC-153 was sufficient to detect acetylated chicken erythrocyte histones (sodium butyrate-treated) using 20 µg total protein, on western blot by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone No.	7F8
Immunogen	Acetylated KLH
Target	Acetylated Lysine
Purification	Protein G Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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N6-Acetyl-L-lysine, N-epsilon-Acetyl-L-lysine, Nepsilon-Acetyllysine, N6-Acetyllysine, Acetyllysine

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Immunohistochemistry analysis using Mouse Anti-Acetylated Lysine Monoclonal Antibody, Clone 7F8. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Acetylated Lysine Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Backskin obtained from transgenic mice.

Western Blot analysis of acetylated lysine showing detection of Acetylated Lysine protein using Mouse Anti-Acetylated Lysine Monoclonal Antibody, Clone 7F8. Primary Antibody: Mouse Anti-Acetylated Lysine Monoclonal Antibody at 1:1000. (1) acetylated BSA (75ng of protein), (2) non-acetylated BSA, and (3) marker.

Quick Database Links

Gene Symbol

Acetylated Lysine

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ICC

Immunocytochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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Immunoprecipitation

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