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Actin, alpha skeletal muscle Mouse Monoclonal Antibody
SKU: orb1227215
Featured
Description
Research Area
Actin Binding Proteins, Actin Crosslinking, Cell Biology, Cytoskeleton, Heart Disease, Signal Transduction, Troponin
Images & Validation
−Item 1 of 4
| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | WB=1:500-2000, IHC-P=1:200-800, IHC-F=1:100-500, IF=1:100-500 |
| Reactivity | Human, Rat |
| Predicted Reactivity | Bovine, Mouse, Sheep |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone No. | 3E9 |
| Immunogen | KLH conjugated synthetic peptide derived from human ACTA1 |
| Target | ACTA1 |
| Molecular Weight | 42 kDa |
| Purification | Affinity purified by Protein G |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−ACTS_HUMAN; Actin, alpha skeletal muscle; alpha-actin-1; ACTA1; ACTA; ASMA; CFTD; CFTD1; CFTDM; MPFD; NEM1; NEM2; NEM3; Actin alpha skeletal muscle; actin, alpha 1, skeletal muscle 1; actin, alpha 1, skeletal muscle; actina; actine; aktin; alpha Actin 1; alpha skeletal muscle Actin; alpha skeletal muscle; alpha-actin; beta cytoskeletal actin; nemaline myopathy type 3.
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25 ug total protein per lane of various lysates probed with Actin, alpha skeletal muscle monoclonal antibody, unconjugated (orb1227215) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Actin, alpha skeletal muscle) Monoclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Actin, alpha skeletal muscle) Monoclonal Antibody, Unconjugated (orb1227215) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Actin, alpha skeletal muscle) Monoclonal Antibody, Unconjugated (orb1227215) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Quick Database Links
Gene Symbol
ACTA1
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
Actin, alpha skeletal muscle Mouse Monoclonal Antibody (orb1227215)
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